Cy3,NHS酯(Cy3) 已用于标记生物分子,用于荧光成像和其他基于荧光的生化分析。它们广泛用于标记肽,蛋白质和寡核苷酸等。Cy3染料在与蛋白质结合后荧光大幅度增强。
图示
图1.将HeLa细胞与(Tubulin +)和(Tubulin-)小鼠抗微管蛋白分别孵育,然后与AAT的Cy3山羊抗小鼠IgG缀合物结合(红色,左);与Jackson 的Cy3缀合物结合(红色 ,右)。 细胞核用Hoechst 33342(Blue,Cat#17530)染色。
参考文献
Basal condensation of Numb and Pon complex via phase transition during Drosophila neuroblast asymmetric division
Authors: Zelin Shan, Yuting Tu, Ying Yang, Ziheng Liu, Menglong Zeng, Huisha Xu, Jiafu Long, Mingjie Zhang, Yu Cai, Wenyu Wen
Journal: Nature Communications (2018): 737
Cube-shaped theranostic paclitaxel prodrug nanocrystals with surface functionalization of SPC and MPEG-DSPE for imaging and chemotherapy
Authors: Fuqiang Guo, Jiajia Shang, Hai Zhao, Kangrong Lai, Yang Li, Zhongxiong Fan, Zhenqing Hou, Guanghao Su
Journal: Colloids and Surfaces B: Biointerfaces (2017)
Light/magnetic hyperthermia triggered drug released from multi-functional thermo-sensitive magnetoliposomes for precise cancer synergetic theranostics
Authors: Yuxin Guo, Yang Zhang, Jinyuan Ma, Qi Li, Yang Li, Xinyi Zhou, Dan Zhao, Hua Song, Qing Chen, Xuan Zhu
Journal: Journal of Controlled Release (2017)
公司简介
西安百萤生物科技有限公司是一家专注从事生物、化学试剂及其试剂盒的高新技术企业;主要以荧光探针,分子探针,核酸/蛋白质标记、细胞检测等技术和产品为研发核心,产品应用于基因合成、细胞检测、活体示踪,抗体标记、新药筛选等领域。